Ribofect cp transfection kit 166t
WebbpSicoR-GSK-3β group. During transfection, 100 μL 1 X riboFECTTM CP buffer was used to dilute 3 μL pSicoR-blank, pSicroR-GSK-3β, miR-NC, and miR-224 inhibitor. After room …
Ribofect cp transfection kit 166t
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Webb1 apr. 2024 · Cells were cultured in a 24-well plate before transfection. When cells grew to 30–50% of confluence, the miRNAs or siRNAs were mixed with riboFECT CP Buffer and riboFECT CP Reagent and incubated for 15 min at room temperature. The mixture was then added to each well and incubated for 48 h. Microinjection of Zygotes With miRNA … Webb18 nov. 2015 · UACC903 cells were transfected with negative control siRNA (100 nM) or target gene siRNA (si LEF-1 or si MITF) (100 nM) using RiboFect CP Transfection Kit (166 T) (RiboBioCo. Ltd. Guangzhou, China) according to the manufacturer’s instructions.
Webb166T 产品描述 ribo FectTM CP转染试剂是一种专门用于核酸分子的转染试剂，极具生物相容性，转染效率高，细胞毒性小，适合各种核酸分子 (如siRNA、miRNA、质粒)的转染 … Webb13 jan. 2024 · siRNA and plasmid transfections The human esophageal carcinoma cells (KYSE150, KYSE450) were grown in 60-mm plates at about 30% confluence and transfected with siRNA duplexes after incubation for 24 h, using the riboFECT CP transfection kit (Ribobio, Guangzhou, China).
WebbAdditionally, to silence the expression of circFAM114A2 and HHIP, two small interfering RNAs (siRNA) were synthesized by RiboBio (China). The transfection of siRNAs, miR-630 mimic, and scramble controls were performed using the riboFECT CP Transfection Kit (Ribobio, C10511-05). All siRNA sequences are shown in Table S1. Webbwherein the double-stranded RNAi agent comprises a sense strand and an antisense strand forming a double stranded region, the antisense strand comprising a region of complementari
Webb18 jan. 2024 · The siRNA oligonucleotides for mouse COL1A1 and siRNA control were purchased from Ribobio. MC3T3‐E1 and Raw264.7 cells were transfected using a RiboFECT CP Transfection Kit (RiboBio), followed by validation of mRNA change at 36 h after transfection using qPCR and protein change at 48 h using western blotting.
Webb16 nov. 2024 · All the transfection experiments were performed using riboFECT CP transfection kit were supplied by Guangzhou Ribobio, China. Western blot and qRT–PCR assays were performed to confirm the effect of AK4 on the expression of miR–199a-3p. The sequences used in this study are as follows: si-AK4: GCCTAATGATGTCCGAGTT 5′ … rosehip oil for scars before and afterWebbRibofecttm Cp Transfection Kit 166t, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … rosehip oil inci nameWebbPrice from $9.99 to $1999.99 ribofect cp transfection kit - by Bioz Stars , 2024-04 86 / 100 stars Buy from Supplier ribofect cp transfection kit ( Thermo Fisher ) 86 Thermo Fisher … store heatmap analysisWebbRibo Fect Tm Cp Transfection Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … rosehip oil from chilehttp://img1.bioon.com/reagent/cpxj3.asp?id=4430241 rosehip oil inciWebb22 okt. 2024 · The cells were transfected in triplicate with each firefly luciferase reporter construct in combination with the Renilla luciferase-based control construct using the riboFECT CP transfection reagent, and both the luciferase activities were measured in the cell extracts 24 h after transfection. store helmet when riding bikeWebb5 aug. 2016 · Mimic, agomir, antagomir, and scrambled (negative control, NC) siRNA sequences as well as a riboFECT CP transfection kit were supplied by Guangzhou RiboBio, China. A GFP-tagged KIF26B overexpression construct (pReciever-M98) was purchased from Genecopia, Guangzhou, China (Catalog No.: EX-E0804-M98-5). rosehip oil in diffuser