2024 Pac5.1/v5-his b

Pac5.1/v5-his b

Author: raeh

August undefined, 2024

WebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。 WebFeb 3, 2024 · The Aag2 cells were transfected by the plasmids of pAc.5.1-Rel1A-V5 / pAc5.1-Rel1B-V5 (1:1 mix) or pAc5.1-STAT-V5 for 48 h, respectively. Then infected the cells with logarithmic-phase E. coli at a final concentration of 0.05 OD 600 for 12 h as described above. Subsequently, the transfected and infected cells were washed twice and collected in ...

MCLAB Products

http://www.biofeng.com/plus/view.php?aid=13720 WebBriefly, the purified cloned vector (pAc5.1/V5-His/Kaz-S) was transfected by use of calcium phosphate (Invitrogen) into 2 × 10 6 to 4 × 10 6 S2 cells/ml in six-well cell culture plates (Costar). The cells were harvested at 48, 72, 96, 120, and 144 h after plasmid transfection. ridgeland high school calendar

Full article: Poly(A) Signals Located near the 5′ End of Genes Are ...

WebDec 12, 2024 · The Drosophila inducible expression system vector, pAc5.1/V5-His B (Invitrogen, Carlsbad, CA), was used to construct plasmid that express protein in Drosophila S2 cells. The diap1 or ntan1 ORF was amplified from fly cDNAs, kindly provided by Dr. Jianquan Ni (Tsinghua University, Beijing, China), by polymerase chain reaction (PCR). WebFeb 15, 2024 · The constructed plasmid pAc5.1/V5- Drsl1 was used to transfect cells with Xtreme GENE HP DNA Transfection Reagent (Roche, USA). Synthetic ds Drsl1 was transfected into cells with EndoFectinTM-Max (GeneCopoeia, USA). For the specific steps of the transfection process, please refer to the previous article of our group ( Ying et al., … WebKEYWORDS pAc5.1 V5-His B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5379) AUTHORS Invitrogen (Life Technologies) TITLE … ridgeland health oak lawn il

Diagnostic Potential of Puumala Virus Nucleocapsid Protein Expressed …

Pac5.1/v5-his b

WebRCASBP-AP (A) (CC#37) 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务：质粒构建基因合成质粒大提. 立刻购买. WebThe lacZ reporter was generated by inserting a PCR fragment of the complete lacZ coding region (amplified from plasmid pAc5.1/V5-His/LacZ [Invitrogen]) in the KpnI and XhoI sites of pAc5.1. In the lacZ reporter the bovine growth hormone gene (BGH) poly(A) signal (PCR amplified from pCDNA3.1 [Invitrogen]) was inserted into the KpnI site ...

Did you know?

WebpAc5.1/V5-His A, B, and C Ac5 forward ACACAAAGCCGCTCCATCAG a-Factor TACTATTGCCAGCATTGCTGC Pichia methanolica expression vector AUG1 forward CAATTTACATCTTTATTTATTAACG AUG1 reverse GAAGAGAAAAACATTAGTTGGC ... http://www.biofeng.com/adjy/zhili/RCASBP-AP-A-CC-37.html

WebpAc5.1 V5-His B. Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His A or pAc5.1/V5-His … WebLarge fragments of DNA (≥ 5 kb) are amenable to cloning in high-copy number vectors, yet at a much lower efficiency. Vector-to-Insert Ratio Optimization of molar concentration ratios of the vector to insert is critical to ensure efficient cloning. Successful cloning ratios may range from 1:1 to 1:10.

WebpAc5.1/V5-His B: Other: Ampicillin: Invitrogen: pAc5.1/V5-His C: Other: Ampicillin: Invitrogen: Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene.

Web丁香通商铺武汉华尔纳生物科技有限公司为您提供细胞库 / 细胞培养、elisa 试剂盒、抗体、试剂、耗材等分类产品11件，全方位满足您的科研实验采购需求

WebFeb 5, 2016 · V5-tagged MsDorsal-RHD, Flag-tagged MsRel2-RHD, V5-tagged MsRel2A and MsRel2B (full-length proteins) were transiently expressed in S2 or Sf9 cells, and cytoplasmic (Cp) and nuclear (Nu)... ridgeland high school girls basketballWebFeb 26, 2007 · The coding sequence for the signal sequence of premembrane and E protein was cloned into the Drosophila expression vector pAc5.1/V5-His. A Drosophila cell line S2 was cotransfected with this construct as well as a … ridgeland high school staffWebpAc5.1 V5-His A Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His B or pAc5.1/V5-His C. … ridgeland high school logoWebComments for pAc5.1/V5-His A 5375 base pairs Ac5 promoter: bases 46-2568 TATA box: bases 2451-2456 Transcription start sites: base 2481, 2482, or 2483 Ac5 forward priming … ridgeland high school soccer fieldWebA biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields ridgeland high school ga greatschoolsWebpBAD His A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核荧光 pQBI63,pMP2444, ... 常见载体引物表 Vector PBAD pACT pAc5.1/V5-His Primer(F) PBAD-F/TRXF T7 Ac5-Profor GAL4-AD-Cfor Primer(R) ... ridgeland high school baseballWebMC Easy Depository™ for DNA Sequencing Guidelines We provide custom with oligonucleotide synthesis service in combination of our DNA sequencing service to better assist our customers. First time primer synthesis order will be fulfilled within 24 hours. ridgeland high school ga school shooting